11/19 pubmed: adipose stem cell

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: adipose stem cell ADIPOSE DERIVED STEM CELL DELIVERY INTO COLLAGEN GELS USING CHITOSAN MICROSPHERES. November 18, 2009 at 8:04 am Related Articles ADIPOSE DERIVED STEM CELL DELIVERY INTO COLLAGEN GELS USING CHITOSAN MICROSPHERES. Tissue Eng Part A. 2009 Nov 16; Authors: Natesan S, Baer DG, Walters TJ, Babu M, Christy R Integration of stem cells to injured tissues requires an appropriate delivery device and scaffolding system. In the present study we have developed an in vitro strategy to load and release adipose derived mesenchymal stem cells (ASC) from chitosan microspheres into a collagen gel scaffold. Porous chitosan microspheres of uniform size and composition were prepared and used as a stem cell carrier. Adipose derived stem cells were allowed to attach to the microspheres and infiltrate through the microsphere pores. The number of viable cells were measured in vitro, using MTT and Calcein AM assays, and showed a proportional increase with seeding density and reached a maximum cell number by 24 hours. The cells inside the microspheres remained metabolically active and viable, could be retrieved from the spheres and maintained expression of stem cell specific markers. Electron microscopic evaluation of the cell-microsphere complex showed that the chitosan microspheres were able to support cell attachment and that the cells had infiltrated into the pores of the microspheres. The ability of the cells to self-renew and differentiate into adipogenic and osteogenic-like precursors indicate that the cells have maintained their multipotency after migration out of the microspheres. To mimic cell delivery into a tissue, ASC-loaded chitosan microspheres were embedded in type-1 collagen scaffold by mixing them with type-1 collagen solution while inducing gelation. By 14 days the cells released into the collagen gel and were able to populate the entire scaffold. When observed through transmission electron microscopy, the cells align along the collagen fibrils with characteristic fibroblast-like morphology. This study provides a model to capture pluripotent stem cells, expand their cell number within a biomaterial scaffold in vitro and deliver within an appropriate matrix to repair damaged tissue. PMID: 19916819 [PubMed - as supplied by publisher] Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation. November 18, 2009 at 8:04 am Related Articles Genotoxic damage of human adipose-tissue derived mesenchymal stem cells triggers their terminal differentiation. Neoplasma. 2009;56(6):542-7 Authors: Altanerova V, Horvathova E, Matuskova M, Kucerova L, Altaner C Human adipose tissue-derived mesenchymal (stromal) stem cells (AT-MSCs) and genetically modified to express cytosine deaminase:uracil phosphoribosyltransferase (CDy-AT-MSCs) were treated with hydrogen peroxide in order to induce DNA damage and subsequently evaluate their genetic stability by single cell gel electrophoresis. Both cells types (parental and transgene modified) did not differ in the sensitivity to DNA breaks induction. Potential tumorigenicity of AT-MSCs and CDy-AT-MSCs was tested by subcutaneous inoculation of cell suspension into flank of immunocompromised mice. Dose of 15x10(6) cells was not found to be tumorigenic in given experimental setup. AT-MSCs, CDy-AT-MSCs and MSCs isolated from human lipoma were treated with chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) in attempts to transform them. Surviving cells after genotoxic stress were not transformed but underwent replicative senescence. Irreparable DNA damage caused triggered adipogenic terminal differentiation, rather than apoptosis induction in all kinds of cells tested. PMID: 19728764 [PubMed - indexed for MEDLINE]   This email was sent to agupta1213+termsc@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.