|  |  |  | | | | | TE-RegenMed-StemCell feed | | | | | | | | | | | | | | | | | | | The selective ablation of inflammation in an acute stage of ischemic stroke may be a new strategy to promote neurogenesis. Med Hypotheses. 2010 Nov 25; Authors: Zhou J, Cheng G, Kong R, Gao DK, Zhang X Ischemic stroke is one of the most common diseases in the world. Pre-clinical studies have proved that stem cell therapy is effective in treating ischemic stroke. But there is a "time window" for stem cell therapy that is only limited in acute/subacute stage after stroke. Meanwhile, ischemic stroke can elicit an immediate neuroinflammatory reaction in the brain, and an uncontrolled inflammatory process in acute/subacute stage will impair survival of stem cells and block repair processes. A selective ablation of harmful inflammation factors can greatly decrease a hostile environment and facilitate neurogenesis. If detrimental factors of inflammation in an acute/subacute stage after an ischemic stroke are suitably handled and more specific immunomodulatory interventions are adopted, neurogenesis in the "time window" will greatly enhanced. PMID: 21112156 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats. Exp Mol Pathol. 2010 Nov 24; Authors: Berardi GR, Rebelatto CK, Tavares HF, Ingberman M, Shigunov P, Barchiki F, Aguiar AM, Miyague NI, Francisco JC, Correa A, Senegaglia AC, Suss PH, Moutinho JA, Sotomaior VS, Nakao LS, Brofman PS Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-d,l-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that received untreated cells (p=0.008). Immunohistochemical reactions showed an increased expression of troponin T-C and von Willebrand factor, and organized vascular units in the infarcted area of tissue transplanted with treated ADSCs. SNAP exposure induced intracellular S-nitrosation, a decreased GSH/GSSG ratio, but did not increase cGMP levels. Collectively, these results indicate that SNAP alters the redox environment of ADSCs, possibly associated with a pre-differentiation state, which may improve cardiac function after transplantation. PMID: 21111728 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Glycoproteomic Analysis of Glioblastoma Stem Cell Differentiation. J Proteome Res. 2010 Nov 29; Authors: He J, Liu Y, Zhu TS, Xie X, Costello MA, Talsma CE, Flack CG, Crowley JG, Dimeco F, Vescovi AL, Fan X, Lubman DM Cancer stem cells are responsible for tumor formation through self-renewal and differentiation into multiple cell types, and thus represent a new therapeutic target for tumors. Glycoproteins play a critical role in determining the fates of stem cells such as self-renewal, proliferation and differentiation. Here we applied a multi-lectin affinity chromatography and quantitative glycoproteomics approach to analyze alterations of glycoproteins relevant to the differentiation of a glioblastoma-derived stem cell line HSR-GBM1. Three lectins including concanavalin A (Con A), wheat germ agglutinin (WGA) and peanut agglutinin (PNA) were used to capture glycoproteins, followed by LC-MS/MS analysis. A total of 73 and 79 high-confidence (FDR < 0.01) glycoproteins were identified from the undifferentiated and differentiated cells, respectively. Label-free quantitation resulted in the discovery of 18 differentially expressed glycoproteins, wherein 9 proteins are localized in the lysosome. All of these lysosomal glycoproteins were up-regulated after differentiation, where their principal function was hydrolysis of glycosyl residues. Protein-protein interaction and functional analyses revealed the active involvement of lysosomes during the process of glioblastoma stem cell differentiation. This work provides glycoprotein markers to characterize differentiation status of glioblastoma stem cells which may be useful in stem-cell therapy of glioblastoma. PMID: 21110520 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats. Exp Mol Pathol. 2010 Nov 24; Authors: Berardi GR, Rebelatto CK, Tavares HF, Ingberman M, Shigunov P, Barchiki F, Aguiar AM, Miyague NI, Francisco JC, Correa A, Senegaglia AC, Suss PH, Moutinho JA, Sotomaior VS, Nakao LS, Brofman PS Stem cell therapy has been considered a promise for damaged myocardial tissue. We have previously shown that S-nitroso-N-acetyl-d,l-penicillamine (SNAP) increases the expression of several muscular markers and VEGF in mesenchymal stem cells, indicating that transplantation of SNAP-treated cells could provide better functional outcomes. Here, we transplanted SNAP-treated adipose tissue-derived stem cells (ADSCs) in rat infarcted myocardium. After 30days, we observed a significant improvement of the ejection fraction in rats that received SNAP-treated ADSCs, compared with those that received untreated cells (p=0.008). Immunohistochemical reactions showed an increased expression of troponin T-C and von Willebrand factor, and organized vascular units in the infarcted area of tissue transplanted with treated ADSCs. SNAP exposure induced intracellular S-nitrosation, a decreased GSH/GSSG ratio, but did not increase cGMP levels. Collectively, these results indicate that SNAP alters the redox environment of ADSCs, possibly associated with a pre-differentiation state, which may improve cardiac function after transplantation. PMID: 21111728 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Stereomask lithography (SML): a universal multi-object micro-patterning technique for biological applications. Lab Chip. 2010 Nov 26; Authors: Zhao S, Chen A, Revzin A, Pan T The advent of biological micro-patterning techniques has given new impetus to many areas of biological research, including quantitative biochemical analysis, tissue engineering, biosensing, and regenerative medicine. Derived from photolithography or soft lithography, current bio-patterning approaches have yet to completely address the needs of out-of-cleanroom, universal applicability, high feature resolution, as well as multi-object placement, though many have shown great promise to precisely pattern one specific biomaterial. In this paper, we present a novel versatile biological lithography technique to achieve integrated multi-object patterning with high feature resolution and high adaptability to various biomaterials, referred to as stereomask lithography (SML). Successive patterning of multiple objects is enabled by using unique three-dimensional masks (i.e., the stereomasks), which lay out current micropatterns while protecting pre-existing biological features on the substrate. Furthermore, high-precision reversible alignment among multiple bio-objects is achieved by adopting a peg-in-hole design between the substrate and stereomasks. We demonstrate that the SML technique is capable of constructing a complex biological microenvironment with various bio-functional components at the single-cell resolution, which to the best of our knowledge has not been realized before. PMID: 21113523 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Preparation and Characterization of Culture of CD146+ Cells from Human Adipose Tissue. Bull Exp Biol Med. 2010 Jul;149(1):113-8 Authors: Rzhaninova AA, Kulikov AV, Spirova IA, Kirienko EE, Volkov AV, Goldshtein DV A method for isolation of homogenous culture of cells expressing CD146 marker from adipose tissue lipoaspirate was developed. The resultant clonogenic cultures retained high proliferative activity, immunophenotype, and morphology after numerous passages. The presence of insulin in the medium served as the selective factor for maintenance of the population phenotype. These cultures effectively differentiate into CD31(+)endothelial cells and can be used in regenerative medicine. PMID: 21113472 [PubMed - in process] | | | | | | | | | | | | | | | | | | | | | | | | | Stem cells and regenerative medicine: accomplishments to date and future promise. Ther Deliv. 2010 Nov;1(5):693-705 Authors: Helmy KY, Patel SA, Silverio K, Pliner L, Rameshwar P More than fifty years has passed since the first allogeneic hematopoietic stem cell transplant in patients, however, the promise of other stem cell populations for tissue replacement and repair remains unachieved. When considering cell-based interventions for personalized medicine, the factors influencing therapeutic success and safety are more complicated than for traditional small-molecule pharmacological agents and protein biologics. Failure to progress personalized stem cell therapies to the clinic has resulted from complications that include an incomplete understanding of stem cell development programs and the diversity of host-donor interactions between patients and in different microenvironments within the same patient. In order to more rapidly extend the use of non-hematopoietic stem cells to the clinic, a better understanding of the different stem cell sources and the implications of their host interactions is required. In this review, we introduce currently available stem cell sources and highlight recent literature that instructs the potential and limitations of their use, with a focus on mesenchymal stem cells. PMID: 21113422 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Interleukin-1β with learning and memory. Neurosci Bull. 2010 Dec;26(6):455-68 Authors: Huang ZB, Sheng GQ Interleukin-1β (IL-1β) is one of the first cytokines ever described. It has long been recognized to play an important role in mediating inflammation and orchestrating the physiological and behavioral adjustments that occur during sickness. Recently, accumulating evidence has indicated that IL-1β also adversely affects cognitive function. Nevertheless, there are also some reports showing no effects or even beneficial effects of IL-1β on learning and memory. The relationship between IL-1β and cognitive impairment has not been clearly elucidated. Here we reviewed the evidence of both negative and positive effects of IL-1β on learning and memory, and the key factors that may affect the effects of IL-1β on learning and memory were discussed. PMID: 21113196 [PubMed - in process] | | | | | | | | | | | | | | | | | | | | | | | | | Development of a High Resolution Purification Method for Precise Functional Characterization of Primitive Human Cord Blood-derived CD34-negative SCID-repopulating Cells. Exp Hematol. 2010 Nov 25; Authors: Ishii M, Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Asano H, Uemura Y, Tsuji T, Fukuhara S, Sonoda Y OBJECTIVE: We have successfully identified human cord blood (CB)-derived CD34-negative (CD34(-)) severe combined immunodeficiency (SCID)-repopulating cells (SRCs) with extensive lympho-myeloid repopulating ability using the intra-bone marrow injection (IBMI) method. In our previous study, a limiting dilution analysis demonstrated the frequency of CD34(-) SRCs in CB-derived 13lineage-negative (Lin(-)) CD34(-) cells to be approximately 1/25,000. In this study, we intended to develop a high resolution purification method in order to obtain highly purified CD34(-) SRCs. MATERIALS AND METHODS: The pooled CB-derived Lin(-) cells were stained with 13 reported Lin monoclonal antibodies (mAbs) and 5 more Lin mAbs, against CD11b, CD33, CD66c, CD45RA, and CD127. Then 18Lin(-)CD34(high), 18Lin(-)CD34(-), and 13Lin(-)CD34(high)CD38(-) cells were sorted by FACS. Stem cell characteristics of these 3 fractions of cells were analyzed by in vitro cultures and in vivo repopulation assays for the evaluation of this new purification method. RESULTS: A limiting dilution analysis demonstrated the frequency of CD34(-) SRCs in these 18Lin(-)CD34(-) cells to be approximately 1/1,000, which is associated with a seeding efficiency 25 times greater than the previous method. All primary recipient NOD/Shi-scid/IL-2Rγc(null) mice that received transplants of only 2 CD34(-) SRCs were highly engrafted with human lympho-myeloid cells at 24 weeks after primary transplantation and showed secondary multilineage repopulating abilities. CONCLUSION: We succeeded to highly purify the CD34(-) SRCs using 18 Lin mAbs and the IBMI technique. This newly developed high resolution purification method is indispensable to precisely characterize a distinct class of primitive human CB-derived CD34(-) hematopoietic stem cells. PMID: 21112372 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | "Positive review for California stem cell agency" – that's the headline on the Nature magazine's Web site concerning the assessment of CIRM by a blue-ribbon panel.
The piece by Alla Katsnelson began:
"The first comprehensive external review of the California Institute for Regenerative Medicine (CIRM) has come to overwhelmingly positive conclusions about the state stem cell agency's progress. | | | | | | | | | | | | | | | | | | | | | | | | | Stereomask lithography (SML): a universal multi-object micro-patterning technique for biological applications. Lab Chip. 2010 Nov 26; Authors: Zhao S, Chen A, Revzin A, Pan T The advent of biological micro-patterning techniques has given new impetus to many areas of biological research, including quantitative biochemical analysis, tissue engineering, biosensing, and regenerative medicine. Derived from photolithography or soft lithography, current bio-patterning approaches have yet to completely address the needs of out-of-cleanroom, universal applicability, high feature resolution, as well as multi-object placement, though many have shown great promise to precisely pattern one specific biomaterial. In this paper, we present a novel versatile biological lithography technique to achieve integrated multi-object patterning with high feature resolution and high adaptability to various biomaterials, referred to as stereomask lithography (SML). Successive patterning of multiple objects is enabled by using unique three-dimensional masks (i.e., the stereomasks), which lay out current micropatterns while protecting pre-existing biological features on the substrate. Furthermore, high-precision reversible alignment among multiple bio-objects is achieved by adopting a peg-in-hole design between the substrate and stereomasks. We demonstrate that the SML technique is capable of constructing a complex biological microenvironment with various bio-functional components at the single-cell resolution, which to the best of our knowledge has not been realized before. PMID: 21113523 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Mesenchymal cells of the decidual tooth pulp: cytophenotype and initial evaluation of possibility of their use in bone tissue engineering. Bull Exp Biol Med. 2010 Jul;149(1):161-6 Authors: Vakhrushev IV, Suzdaltseva YG, Burunova VV, Karalkin PA, Lupatov AY, Yarygin KN Cultures of mesenchymal cells from human decidual tooth pulp were derived. The phenotype and capacity to osteogenic and adipogenic differentiation of these cells are close to those of bone marrow mesenchymal stem cells. Decidual tooth pulp mesenchymal cells populate biodegraded polylactide scaffolds and hence, can be used for the creation of tissue engineering transplants for bone defect repair. Storage of decidual tooth pulp mesenchymal cells in the stem cell cryobanks together with umbilical blood will appreciably extent the periods of age for collection of juvenile autologous stem cells for use throughout the life span. PMID: 21113481 [PubMed - in process] | | | | | | | | | | | | | | | | | | | | | | | | | Biocompatibility of tissue engineering constructions from porous polylactide carriers obtained by the method of selective laser sintering and bone marrow-derived multipotent stromal cells. Bull Exp Biol Med. 2010 Jul;149(1):148-53 Authors: Bukharova TB, Antonov EN, Popov VK, Fatkhudinov TKh, Popova AV, Volkov AV, Bochkova SA, Bagratashvili VN, Gol'dshtein DV We studied the biocompatibility of porous polylactide carrier matrices obtained by means of surface selective laser sintering. Carrier matrices had no cytotoxic activity, but maintained adhesion and proliferation of cells. Subcutaneous transplantation of tissue engineering constructions from these carriers and bone marrow-derived multipotent stromal cells did not cause the inflammatory response and pathological changes in rats. The conditions for organotypic regeneration were provided at the site of transplantation (high degree of blood supply and considerable amount of immature precursor cells). PMID: 21113479 [PubMed - in process] | | | | | | | | | | | | | | | | | | | | | | | | | Scaffold Sheet Design Strategy for Soft Tissue Engineering. Nat Mater. 2010 Feb 24;3(2):1375-1389 Authors: Tran RT, Thevenot P, Zhang Y, Gyawali D, Tang L, Yang J Creating heterogeneous tissue constructs with an even cell distribution and robust mechanical strength remain important challenges to the success of in vivo tissue engineering. To address these issues, we are developing a scaffold sheet tissue engineering strategy consisting of thin (∼200 μm), strong, elastic, and porous crosslinked urethane-doped polyester (CUPE) scaffold sheets that are bonded together chemically or through cell culture. Suture retention of the tissue constructs (four sheets) fabricated by the scaffold sheet tissue engineering strategy is close to the surgical requirement (1.8 N) rendering their potential for immediate implantation without a need for long cell culture times. Cell culture results using 3T3 fibroblasts show that the scaffold sheets are bonded into a tissue construct via the extracellular matrix produced by the cells after 2 weeks of in vitro cell culture. PMID: 21113339 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Clinical application of human mesenchymal stromal cells for bone tissue engineering. Stem Cells Int. 2010;2010:215625 Authors: Chatterjea A, Meijer G, van Blitterswijk C, de Boer J The gold standard in the repair of bony defects is autologous bone grafting, even though it has drawbacks in terms of availability and morbidity at the harvesting site. Bone-tissue engineering, in which osteogenic cells and scaffolds are combined, is considered as a potential bone graft substitute strategy. Proof-of-principle for bone tissue engineering using mesenchymal stromal cells (MSCs) has been demonstrated in various animal models. In addition, 7 human clinical studies have so far been conducted. Because the experimental design and evaluation parameters of the studies are rather heterogeneous, it is difficult to draw conclusive evidence on the performance of one approach over the other. However, it seems that bone apposition by the grafted MSCs in these studies is observed but not sufficient to bridge large bone defects. In this paper, we discuss the published human clinical studies performed so far for bone-tissue regeneration, using culture-expanded, nongenetically modified MSCs from various sources and extract from it points of consideration for future clinical studies. PMID: 21113294 [PubMed - in process] | | | | | | | | | | | | | | | | | | | | | | | | | Integration of multiple cell-matrix interactions into alginate scaffolds for promoting cardiac tissue regeneration. Biomaterials. 2010 Nov 26; Authors: Sapir Y, Kryukov O, Cohen S Cardiac tissue engineering aims to repair damaged myocardial tissues by applying heart patches created in vitro. Herein, we explored the possible role of a combination of two matrix-attached peptides, the adhesion peptide G(4)RGDY and heparin-binding peptide G(4)SPPRRARVTY (HBP) in cardiac tissue regeneration. Neonatal rat cardiac cells were seeded into unmodified, single peptide or double peptide-attached alginate scaffolds, all having the same physical features of porosity, hydrogel forming and matrix stiffness. The cardiac tissue developed in the HBP/RGD-attached scaffolds revealed the best features of a functional muscle tissue, as judged by all studied parameters, i.e., immunostaining of cardiac cell markers, histology, western blot of protein expressions and metabolic activity. By day 7, well-developed myocardial fibers were observed in these cell constructs. At 14 days the HBP/RGD-attached constructs presented an isotropic myofiber arrangement, while no such arrangement was seen in the other constructs. The expression levels of α-actinin, N-cadherin and Connexin-43, showing preservation and an increase in Connexin-43 expression (Cx-43) with time, further supported the formation a contractile muscle tissue in the HBP/RGD-attached scaffolds. Collectively, the attachment of combinatorial peptides representing different signaling in ECM-cell interactions proved to play a key role, contributing to the formation of a functional cardiac muscle tissue, in vitro. PMID: 21112626 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | Evaluation of a hydrogel-fiber composite for ACL tissue engineering. J Biomech. 2010 Nov 24; Authors: Freeman JW, Woods MD, Cromer DA, Ekwueme EC, Andric T, Atiemo EA, Bijoux CH, Laurencin CT The anterior cruciate ligament (ACL) is necessary for normal knee stability and movement. Unfortunately the ACL is also the most frequently injured ligament of the knee with severe disruptions requiring surgical intervention. In response to this, tissue engineering has emerged as an option for ACL replacement and repair. In this study we present a novel hydrogel-fibrous scaffold as a potential option for ACL replacement. The scaffold was composed of PLLA fibers, in a previously evaluated braid-twist structure, combined with a polyethylene glycol diacrylate (PEGDA) hydrogelto improve viscoelastic properties. Both hydrogel concentration (10%, 15%, and 20%) and amount of hydrogel (soaking the fibrous scaffold in hydrogel solution or encasing the scaffold in a block of hydrogel) were evaluated. It was found that the braid-twist scaffold had a greater porosity and larger number of pores above 100μm than braided scaffolds with the same braiding angle. After testing for their effects on swelling, fiber degradation, and protein release, as well as viscoelastic and tensile testing (when combined with fibrous scaffolds), it was found that the composite scaffold soaked in 10% hydrogel had the best chemical release and mechanical properties. The optimized structure behaved similarly to natural ligament in tension with the addition of the hydrogel decreasing the ultimate tensile stress (UTS), but the UTS was still comparable to natural ACL. In addition, cellular studies showed that the hydrogel-PLLA fiber composite supported fibroblast growth. PMID: 21111422 [PubMed - as supplied by publisher] | | | | | | | | | |  | |  | |  |
