| Promotion of human adipose-derived stem cell proliferation mediated by exogenous nucleosides. Cell Biol Int. 2010 Jun 3; Authors: RodrÃguez-Serrano F, Alvarez P, Caba O, Picón M, Marchal JA, Perán M, Prados J, Melguizo C, Rama AR, Boulaiz H, Aránega A Adult stem cells are becoming the best option for regenerative medicine because they have low tumorigenic potential and permit autologous transplantation, even without in vitro culture. Our objectives were to evaluate the effects of exogenous nucleosides on the proliferation of human adipose-derived stem cells (hASCs), with or without co-treatment with 5-azacytidine (5-aza), and to analyze the expression of lamin A/C during cardiomyocyte differentiation of these cells. We isolated hASCs from human lipoaspirates that were positive for mesenchymal stem cell markers. We found that 5-aza induces a dose-dependent inhibition of hASC proliferation (IC50: 5.37muM), whereas exogenous nucleosides significantly promote the proliferation of hASCs and partially revert the antiproliferative effect of the drug. Multipotentiality of isolated hASCs was confirmed by adipogenic, osteogenic, and cardiomyogenic induction. 5-aza-induced cells expressed cardiac troponins I and T and myosin light chain 2 myocardial markers that were directly correlated with lamin A/C expression. Our results support the importance of the nucleoside supplementation of media to improve conditions for the expansion and maintenance of hASCs in culture. In addition, the quantification of lamin A/C expression appears to be a good marker for the characterization of cardiomyocyte differentiation of stem cells that has rarely been used. PMID: 20522021 [PubMed - as supplied by publisher] | 
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