10/30 pubmed: adipose stem cell

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: adipose stem cell Adhesion, Migration, and Mechanics of Human Adipose Tissue Derived Stem Cells on Silk Fibroin-Chitosan Matrix. October 29, 2009 at 7:01 am Adhesion, Migration, and Mechanics of Human Adipose Tissue Derived Stem Cells on Silk Fibroin-Chitosan Matrix. Acta Biomater. 2009 Oct 24; Authors: Altman AM, Gupta V, Ríos CN, Alt EU, Mathur AB Silk fibroin-chitosan (SFCS) scaffold is a naturally-derived biocompatible matrix with potential reconstructive surgical applications. In this study, human adipose-derived mesenchymal stem cells (ASCs) were seeded on SFCS scaffolds and cell attachment was characterized by fluorescence, confocal, time-lapse, atomic force, and scanning electron microscopy (SEM). Adhesion of ASCs on SFCS was 39.4 +/- 4.8% at 15 minutes, increasing to 92.8 +/- 1.5% at 120 minutes. ASC adhered at regions of architectural complexity and infiltrate into three-dimensional scaffold. Time-lapse confocal studies indicated a mean ASC speed on SFCS of 18.47 +/- 2.7 mum/hr and a mean persistence time of 41.4 +/- 9.3 minutes over a 2.75-hour study period. Cytokinetic and SEM studies demonstrated ASC-ASC interaction via microvillus extensions. The apparent elastic modulus was significantly higher (p<0.0001) for ASCs seeded on SFCS (69.0 +/- 9.0 kPa) than on glass (6.1 +/- 0.4 kPa). Also, cytoskeleton F-actin fiber density was higher (p<0.05) for ASC seeded on SFCS (0.42 +/- 0.02 fibers/mum) than on glass-seeded controls (0.24 +/- 0.03 fibers/mum). Hence, SFCS scaffold facilitates mesenchymal stem cell attachment, migration, 3-dimensional infiltration, and cell-cell interaction. This study showed the potential use of SFCS as a local carrier for autologous stem cells for reconstructive surgery application. PMID: 19861180 [PubMed - as supplied by publisher] Delta sleep-inducing peptide and glucocorticoid-induced leucine zipper: potential links between circadian mechanisms and obesity? October 29, 2009 at 7:01 am Delta sleep-inducing peptide and glucocorticoid-induced leucine zipper: potential links between circadian mechanisms and obesity? Obes Rev. 2009 Nov;10 Suppl 2:46-51 Authors: Gimble JM, Ptitsyn AA, Goh BC, Hebert T, Yu G, Wu X, Zvonic S, Shi XM, Floyd ZE As the obesity pandemic has accelerated, investigators have begun to explore alternative mechanisms linking circadian biology and sleep to adipose tissue metabolism and obesity. This manuscript reviews recent findings in murine and human models demonstrating the oscillatory expression of the mRNAs encoding the core circadian regulatory proteins in adipose tissue. Comparative transcriptomic analyses of circadian oscillating genes have been used to identify the 'delta sleep-inducing peptide immunoreactor', also known as 'glucocorticoid-induced leucine zipper (GILZ)', as a potential link in this chain. The GILZ gene has been found to differentially regulate stromal stem cell adipogenic and osteogenic differentiation in a reciprocal manner. In adipose and other metabolically active tissues, the circadian oscillation of GILZ expression is subject to entrainment by external stimuli. Together, these observations suggest that GILZ is an attractive candidate for future studies evaluating the role of circadian mechanisms in adipose tissue physiology and pathology. PMID: 19849801 [PubMed - in process]   This email was sent to agupta1213+termsc@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.