6/10 pubmed: adipose stem cell

pubmed: adipose stem cell [Autotransplantation of multipotent stromal cells from adipose tissue as a method of stimulation of angiogenesis in the limb ishemia in experiment] June 9, 2010 at 6:45 AM Related Articles [Autotransplantation of multipotent stromal cells from adipose tissue as a method of stimulation of angiogenesis in the limb ishemia in experiment] Klin Khir. 2010 Jan;(1):46-50 Authors: Dombrovs'kyÄ­ DB The objective of research conduction for new methods of indirect revascularization of the limbs is actual because of cases existed of impossible performance of operative reconstructive interventions on arteries for the limbs ischemia. Adipose tissue constitutes the accessible and sufficient source of multipotent cells. Experimental investigations on laboratory animals were done for the limb ischemia simulation. Using histological investigations and electronic microscopy the efficacy of autotransplantation of multipotent stromal cells from adipose tissue for angiogenesis stimulation de novo in the limb ischemia was proved. PMID: 20469695 [PubMed - indexed for MEDLINE]   Towards an intraoperative engineering of osteogenic and vasculogenic grafts from the stromal vascular fraction of human adipose tissue. June 9, 2010 at 6:45 AM Related Articles Towards an intraoperative engineering of osteogenic and vasculogenic grafts from the stromal vascular fraction of human adipose tissue. Eur Cell Mater. 2010;19:127-35 Authors: Müller AM, Mehrkens A, Schäfer DJ, Jaquiery C, Güven S, Lehmicke M, Martinetti R, Farhadi I, Jakob M, Scherberich A, Martin I Grafts generated by cultivation of progenitor cells from the stromal vascular fraction of human adipose tissue have been proven to have osteogenic and vasculogenic properties in vivo. However, in vitro manufacture of such implants is challenged by complex, impractical and expensive processes, and requires implantation in a separate surgery. This study investigates the feasibility of an intraoperative approach to engineer cell-based bone grafts with tissue harvest, cell isolation, cell seeding onto a scaffold and subsequent implantation within a few hours. Freshly isolated adipose tissue cells from a total of 11 donors, containing variable fractions of mesenchymal and endothelial progenitors, were embedded at different densities in a fibrin hydrogel, which was wrapped around bone substitute materials based on beta-tricalcium phosphate (ChronOS), hydroxyapatite (Engipore), or acellular xenograft (Bio-Oss). The resulting constructs, generated within 3 hours from biopsy harvest, were immediately implanted ectopically in nude mice and analysed after eight weeks. All explants contained blood vessels formed by human endothelial cells, functionally connected to the recipient's vasculature. Human origin cells were also found within osteoid structures, positively immunostained for bone sialoprotein and osteocalcin. However, even with the highest loaded cell densities, no frank bone tissue was detected, independently of the material used. These results provide a proof-of-principle that an intraoperative engineering of autologous cell-based vasculogenic bone substitutes is feasible, but highlight that - in the absence of in vitro commitment--additional cues (e.g., low dose of osteogenic factors or orthotopic environmental conditions) are likely needed to support complete osteoblastic cell differentiation and bone tissue generation. PMID: 20198567 [PubMed - indexed for MEDLINE]   This email was sent to agupta1213+termsc@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064