6/23 pubmed: adipose stem cell

pubmed: adipose stem cell Pre-transplant C-peptide Level Predicts Early Post-transplant Diabetes Mellitus and Impacts Survival After Allogeneic Stem Cell Transplant. June 22, 2010 at 8:12 AM Pre-transplant C-peptide Level Predicts Early Post-transplant Diabetes Mellitus and Impacts Survival After Allogeneic Stem Cell Transplant. Biol Blood Marrow Transplant. 2010 Jun 15; Authors: Griffith ML, Jagasia M, Misfeldt AA, Chen H, Engelhardt BG, Kassim A, Savani BN, Survant M, Jagasia SM Post-transplant diabetes mellitus (PTDM) is a frequent and important complication after allogeneic stem cell transplantation (allo-SCT) due to its negative impact on cardiovascular health. Risk factors for PTDM are not well defined. We conducted a prospective study to investigate the risk factors and incidence for PTDM in the first 100 days after allo-SCT. Eighty-four patients completed the study and 60% developed PTDM. In a multivariate logistic regression model, pre-transplant c-peptide level (>3.6 ng/mL; OR=5.9, 95% confidence interval (CI) 1.77-20.22, P=0.004), unrelated donor allo-SCT (OR=4.3, 95% CI 1.34-14.2, P=0.014), and peak steroid dose >1 mg/kg/day (OR= 5.09, 95% CI 1.19-23.2, P=0.035) were identified as independent predictors of PTDM. In addition, overall survival (OS) was inferior for patients with PTDM (mean survival 2.26 years vs. 2.7 years, P =0.021). Pre-transplant c-peptide level greater than the cohort median (>3.6 ng/mL) was also associated with inferior OS (mean 1.7 years vs. 2.9 years, P=0.012). In multivariate Cox proportional hazards model, high risk disease (HR=2.34, 95% CI 1.09-5.28, P=0.029) and pre-transplant c-peptide level >3.6 ng/mL (HR=1.05, 95% CI 1.01-1.09, P=0.013) were independent predictors of OS when adjusted for systemic steroids and regimen intensity. We suspect that diabetes mellitus in the immediate post-transplant period may be mediated via an inflammatory pathway that contributes to insulin resistance in the host adipose tissue. Our study is the first to report the risk factors of early PTDM in patients undergoing allo-SCT and identifies pre-transplant c-peptide as an independent predictor of diabetes and survival. PMID: 20561594 [PubMed - as supplied by publisher]   Injections of Adipose Tissue-Derived Stem Cells and Stem Cell Lysate Improve Recovery of Erectile Function in a Rat Model of Cavernous Nerve Injury. June 22, 2010 at 8:12 AM Injections of Adipose Tissue-Derived Stem Cells and Stem Cell Lysate Improve Recovery of Erectile Function in a Rat Model of Cavernous Nerve Injury. J Sex Med. 2010 Jun 17; Authors: Albersen M, Fandel TM, Lin G, Wang G, Banie L, Lin CS, Lue TF ABSTRACT Introduction. Erectile dysfunction (ED) remains a major complication after radical prostatectomy. The use of adipose tissue-derived stem cells (ADSCs) has shown promising results for the treatment of ED. However, the mechanisms of action for stem cell therapy remain controversial, with increasing evidence pointing to paracrine pathways. Aim. To determine the effects and to identify the mechanism of action of ADSC and ADSC-derived lysate in a rat model of cavernous nerve (CN) crush injury. Methods. Thirty-two male Sprague-Dawley rats were randomly divided into four equal groups: one group underwent sham operation, while three groups underwent bilateral CN crush. Crush-injury groups were treated at the time of injury with intracavernous injection of ADSC, lysate, or vehicle only (injured controls). Erectile function was assessed by CN electrostimulation at 4 weeks. Penile tissue was collected for histology. Main Outcome Measures. Intracavernous pressure increase upon CN stimulation; neuronal nitric oxide synthase (nNOS) content in the dorsal penile nerve; smooth muscle content, collagen content, and number of apoptotic cells in the corpus cavernosum. Results. Both ADSC and lysate treatments resulted in significant recovery of erectile function, as compared with vehicle treatment. nNOS content was preserved in both the ADSC and lysate group, with significantly higher expression compared with vehicle-treated animals. There was significantly less fibrosis and a significant preservation of smooth muscle content in the ADSC and lysate groups compared with injured controls. The observed functional improvement after lysate injection supports the hypothesis that ADSCs act through release of intracellular preformed substances or by active secretion of certain biomolecules. The underlying mechanism of recovery appears to involve neuron preservation and cytoprotection by inhibition of apoptosis. Conclusions. Penile injection of both ADSC and ADSC-derived lysate can improve recovery of erectile function in a rat model of neurogenic ED. Albersen M, Fandel TM, Lin G, Wang G, Banie L, Lin CS, and Lue TF. Injections of adipose tissue-derived stem cells and stem cell lysate improve recovery of erectile function in a rat model of cavernous nerve injury. J Sex Med **;**:**-**. PMID: 20561166 [PubMed - as supplied by publisher]   This email was sent to agupta1213+termsc@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064