10/31 pubmed: adipose stem cell

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: adipose stem cell Reduction of Neu5GC Xenoantigen on Human ADSC/MSCs lead to Them as Safer and More Useful Cell Sources for Realizing Various Stem Cell Therapies. October 30, 2009 at 10:14 am Related Articles Reduction of Neu5GC Xenoantigen on Human ADSC/MSCs lead to Them as Safer and More Useful Cell Sources for Realizing Various Stem Cell Therapies. Tissue Eng Part A. 2009 Oct 28; Authors: Komoda H, Okura H, Lee CM, Sougawa N, Iwayama T, Hashikawa T, Saga A, Yamamoto A, Ichinose A, Murakami S, Sawa Y, Matsuyama A Adipose tissue is an attractive source for somatic stem cell therapy. Currently, human ADSC/MSCs are usually cultured with fetal bovine serum (FBS). Recently, however, not only human embryonic stem cell lines cultured on mouse feeder cells but also bone marrow derived hMSCs cultured with FBS were reported to express N-glycolylneuraminic acid (Neu5Gc) xenoantigen. Human serum contains high titers of natural preformed antibodies against Neu5Gc. We studied the presence of Neu5Gc on hADSC/MSCs cultured with FBS and human immune response mediated by Neu5Gc. Our data indicated that hADSC/MSCs cultured with FBS expressed Neu5Gc and human natural preformed antibodies could bind to hADSC/MSCs. However, hADSC/MSCs express complement regulatory proteins such as CD46, CD55, and CD59 and is largely resistant to complement-mediated cytotoxity (CMC). hADSC/MSCs cultured with FBS could be injured by antibody dependent cell-mediated cytotoxicity (ADCC) mechanism. Furthermore, human monocyte derived macrophages could phagocytose hADSC/MSCs cultured with FBS and this phagocytosis activity increased in the presence of human serum. Culturing the hADSC/MSCs with heat-inactivated human serum for a week could markedly reduce Neu5Gc on hADSC/MSCs and prevented immune responses mediated by Neu5Gc, such as human natural preformed antibodies binding, ADCC, and phagocytosis. Adipogenic and osteogenic differentiation potential of hADSC/MSCs cultured with heat-inactivated human serum was not less than those cultured with FBS. For stem cell therapies based on hADSC/MSCs to be realized, hADSC/MSCs that presented Neu5Gc on their cell surfaces after exposure to FBS should be clean up to be rescued from xenogenic rejection. PMID: 19863253 [PubMed - as supplied by publisher]   This email was sent to agupta1213+termsc@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.