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TERMSC In situ functionalization of wet-spun fibre meshes for bone tissue engineering. July 25, 2010 at 10:42 PM Related Articles In situ functionalization of wet-spun fibre meshes for bone tissue engineering. J Tissue Eng Regen Med. 2010 Jul 23; Authors: Leonor IB, Rodrigues MT, Gomes ME, Reis RL Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si--OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si--OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si--OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si--OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo. Copyright (c) 2010 John Wiley & Sons, Ltd. PMID: 20653041 [PubMed - as supplied by publisher]   Temperature as a Single On-Off Parameter Controlling Nanoparticles Growing, Stabilization and Fast Disentanglement. July 25, 2010 at 10:42 PM Related Articles Temperature as a Single On-Off Parameter Controlling Nanoparticles Growing, Stabilization and Fast Disentanglement. Adv Mater. 2010 Jul 22; Authors: López-Pérez PM, da Silva RM, Pashkuleva I, Román JS, Reis RL PMID: 20652903 [PubMed - as supplied by publisher]   Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes. July 25, 2010 at 10:42 PM Related Articles Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes. Osteoarthritis Cartilage. 2010 Jul 19; Authors: Buckley CT, Vinardell T, Kelly DJ BACKGROUND: For current tissue engineering or regenerative medicine strategies, chondrocyte- or mesenchymal stem cell (MSC)-seeded constructs are typically cultured in normoxic conditions (20% oxygen). However, within the knee joint capsule a lower oxygen tension exists. OBJECTIVE: The objective of this study was to investigate how chondrocytes and infrapatellar fad pad derived MSCs will respond to a low oxygen (5%) environment in 3D agarose culture. Our hypothesis was that culture in a low oxygen environment (5%) will enhance the functional properties of cartilaginous tissues engineered using both cell sources. EXPERIMENTAL DESIGN: Cell-encapsulated agarose hydrogel constructs (seeded with chondrocytes or infrapatellar fat pad derived MSCs) were prepared and cultured in a chemically defined serum-free medium in the presence (chondrocytes and MSCs) or absence (chondrocytes only) of transforming growth factor-beta3 (TGF-beta3) in normoxic (20%) or low oxygen (5%) conditions for 42 days. Constructs were assessed at days 0, 21 and 42 in terms of mechanical properties, biochemical content and histologically. RESULTS: Low oxygen tension (5%) was observed to promote extracellular matrix production by chondrocytes cultured in the absence of TGF-beta3, but was inhibitory in the presence of TGF-beta3. In contrast, a low oxygen tension enhanced chondrogenesis of infrapatellar fat pad constructs in the presence of TGF-beta3, leading to superior mechanical functionality compared to chondrocytes cultured in identical conditions. CONCLUSIONS: Extrapolating the results of this study to the in vivo setting, it would appear that joint fat pad derived MSCs may possess a superior potential to generate a functional repair tissue in low oxygen tensions. However, in the context of in vitro cartilage tissue engineering, chondrocytes maintained in normoxic conditions in the presence of TGF-beta3 generate the most mechanically functional tissue. PMID: 20650328 [PubMed - as supplied by publisher]   A Matter for CIRM to Ponder: California's Visceral Reaction to High Salaries for Public Servants July 25, 2010 at 6:03 PM   This email was sent to regenmd@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064