7/20 TE-RegenMed-StemCell feed

TE-RegenMed-StemCell feed UC Davis Scientist Praises CIRM Appeals Change July 19, 2010 at 5:33 PM A stem cell researcher at UC Davis today said a change in the CIRM grant appeals procedure makes "a lot of sense." Writing on his blog in regard to "extraordinary petitions," Paul Knoepfler said, "I think the proposed change makes a lot of sense and would greatly improve the process. Sometimes the reasons in the petitions are clearly not meritorious and as it now stands, they end up wasting   Whither Weschler-Reya? A $6 Million Stem Cell Tale July 19, 2010 at 3:11 PM Is a high-flying stem cell researcher from Duke coming to California to claim his $6 million grant from the state's $3 billion stem cell agency? Can the prestigious Sanford-Burnham Institute in tony La Jolla, Ca., lure him and his wife away from their spots at the Gothic Wonderland, as Duke University is sometimes known? The answers to these and other questions are still a mystery despite a   CIRM Finally Discloses Grant Appeals Change Proposals July 19, 2010 at 10:03 AM The California stem cell agency early today belatedly posted a two-page memo on proposed changes in how it will deal with appeals by scientists whose grant applications have been rejected by reviewers. The information comes only one business day before a meeting of the CIRM directors' Science Subcommittee to consider alterations in the appeals process. The changes deal with an issue that has   Characterization of hematopoietic potential of mesenchymal stem cells. July 19, 2010 at 12:15 AM Related Articles Characterization of hematopoietic potential of mesenchymal stem cells. J Cell Physiol. 2010 Jul 15; Authors: Freisinger E, Cramer C, Xia X, Murthy SN, Slakey DP, Chiu E, Newsome ER, Alt EU, Izadpanah R Mesenchymal and hematopoietic tissues are important reservoirs of adult stem cells. The potential of tissue resident mesenchymal stem cells (MSCs) to differentiate into cells of mesodermal and ectodermal lineages has been reported previously. We examined the hypothesis that adherent adipose tissue resident mesenchymal stem cells (ASCs) are capable of generating cells with hematopoietic characteristics. When cultured in differentiation media, clonally isolated ASCs develop into cells with hematopoietic attributes. The differentiated ASCs (HD) express early hematopoietic (c-kit, PROM1, CD4) as well as monocyte/macrophage markers (CCR5, CD68, MRC1, CD11b, CSF1R). Additionally, HD cells display functional characteristics of monocyte/macrophages such as phagocytosis and enzymatic activity of alpha-Naphthyl Acetate Esterase. HD cells are also responsive to stimulation by IL-4 and LPS as shown by increased CD14 and HLA-DRB1 expressions and release of IL-2, IL10, and TNF. Taken together, this study characterizes the potential of ASCs to generate functional macrophages in vitro, and therefore paves way for their possible use in cell therapy applications. J. Cell. Physiol. (c) 2010 Wiley-Liss, Inc. PMID: 20635396 [PubMed - as supplied by publisher]   Limiting-dilution transplantation assays in mammary stem cell studies. July 19, 2010 at 12:15 AM Related Articles Limiting-dilution transplantation assays in mammary stem cell studies. Methods Mol Biol. 2010;621:29-47 Authors: Illa-Bochaca I, Fernandez-Gonzalez R, Shelton DN, Welm BE, Ortiz-de-Solorzano C, Barcellos-Hoff MH Mammary reconstitution assays can be used to measure the stem cell frequency within an epithelial population by transplanting increasingly diluted single-cell preparations of the population of interest. There are fundamental steps in the single-cell isolation protocol which are directly related to the number of single epithelial cells obtained. Once single-cell suspensions have been obtained, serial dilutions are prepared and transplanted into the cleared fat pads of the host mice. After 8-10 weeks, the transplanted fat pads are reevaluated for the presence of epithelial outgrowths. Based on the frequency of no outgrowth for each one of the transplanted dilutions, it is possible to estimate the frequency of mammary repopulating cells present in a given cell population. Here, we give details on how to carry out all these steps. PMID: 20405357 [PubMed - indexed for MEDLINE]   This email was sent to regenmd@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064