1/17 RegenMD

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. RegenMD Conserved expression patterns predict microRNA targets. January 16, 2010 at 1:35 AM Related Articles Conserved expression patterns predict microRNA targets. PLoS Comput Biol. 2009 Sep;5(9):e1000513 Authors: Ritchie W, Rajasekhar M, Flamant S, Rasko JE microR! NAs (miRNAs) are major regulators of gene expression and thereby modulate many biological processes. Computational methods have been instrumental in understanding how miRNAs bind to mRNAs to induce their repression but have proven inaccurate. Here we describe a novel method that combines expression data from human and mouse to discover conserved patterns of expression between orthologous miRNAs and mRNA genes. This method allowed us to predict thousands of putative miRNA targets. Using the luciferase reporter assay, we confirmed 4 out of 6 of our predictions. In addition, this method predicted many miRNAs that act as expression enhancers. We show that many miRNA enhancer effects are mediated through the repression of negative transcriptional regulators and that this effect could be as common as the widely reported repression activity of miRNAs. Our findings suggest that the indirect enhancement of gene expression by miRNAs could be an important component of miRNA regulation! that has been widely neglected to date. PMID: 19779543! [PubMed - indexed for MEDLINE]   This email was sent to regenmd@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.