1/23 pubmed: adipose stem cell

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: adipose stem cell Bladder reconstruction with adipose-derived stem cell-seeded bladder acellular matrix grafts improve morphology composition. January 22, 2010 at 3:22 PM Related Articles Bladder reconstruction with adipose-derived stem cell-seeded bladder acellular matrix grafts improve morphology composition. World J Urol. 2010 Jan 21; Authors: Zhu WD, Xu YM, Feng C, Fu Q, Song LJ, Cui L PURPOSE: To assess the feasibility of seeding adipose-derived stem cells (ADSCs) onto bladder acellular matrix grafts (BAMGs) for bladder reconstruction in a rabbit model. METHODS: Autologous ADSCs were isolated, expanded and identified by flow cytometry. In the experimental group, ADSCs were seeded onto BAMGS for reconstructing bladder defects in 12 male rabbits. Unseeded BAMGs were used for bladder reconstruction in the control group of 12 rabbits. Cystography was performed at 4, 12 and 24 weeks after grafts implantation. Following cystography, the animals were killed and grafts were harvested; H&E and immunohistochemical staining were performed with cytokeratin AE1/AE3, smooth muscle alpha-actin and S-100 markers. RESULTS: Flow cytometry demonstrated that the ADSCs expressed CD90, CD44, CD105, CD166 and CD34, but not CD45 or CD106. The cells demonstrated good biocompatibility with BAMGs. At 24 weeks, in the experimental group, the reconstructed bladders rea! ched a mean volume of 94.68 +/- 3.31% of the pre-cystectomy bladder capacity. Complete regeneration of smooth muscle and nerve tissue was evident. Regenerated SMCs, urothelium and nerve cells stained positively for alpha-smooth muscle actin, AE1/AE3 and S-100. In the control group, the mean bladder volume was 69.33 +/- 5.05% of the pre-cystectomy volume; histologically, the control group was characterized by multi-layered urothelium without evidence for organized muscle or nerve tissue. CONCLUSIONS: These data demonstrate that seeding ADSCs onto BAMGs promote regeneration of smooth muscle and nervous tissue regeneration in a rabbit model. This compound graft was more suitable for bladder reconstruction than BAMG alone. PMID: 20091038 [PubMed - as supplied by publisher]   Effect of varied ionic calcium on human adipose-derived stem cell mineralization. January 22, 2010 at 3:22 PM Related Articles Effect of varied ionic calcium on human adipose-derived stem cell mineralization. Tissue Eng Part A. 2010 Jan 20; Authors: McCullen S, Zhan J, Onorato M, Bernacki S, Loboa E Human adipose-derived stem cells (hASCs) are a relatively abundant and accessible stem cell source with multilineage differentiation capability and have great potential for bone tissue engineering applications. The success of bone tissue engineering is intimately linked with the production of a mineralized matrix that mimics the natural mineral present within native bone. In this study, we examined the effects of ionic calcium levels of 1.8 (normal concentration in cell culture medium), 8, and 16 mM on hASCs seeded in both 2D monolayer and 3D electrospun scaffolds and cultured in either complete growth (CGM) or osteogenic differentiation medium (ODM).. The impact of calcium supplementation on hASC viability, proliferation, and mineral deposition were determined. Human ASCs remained viable for all experimental treatments. Human ASC proliferation increased with the addition of 8 mM Ca2+ CGM, but decreased for the 16 mM Ca2+ CGM treatment. Materials deposited by hASC! s were analyzed using four techniques: 1) histological staining with Alizarin Red S, 2) calcium quantification, 3) Fourier transform infrared spectroscopy (FTIR), and 4) wide angle x-ray diffraction (XRD). Mineral deposition was significantly enhanced under both growth and osteogenic medium conditions by increasing extracellular Ca2+. The greatest mineral deposition occurred in the osteogenic differentiating medium 8 mM Ca2+ treatment group. FTIR analysis indicated that elevated calcium concentrations of 8 mM Ca2+ significantly increased both PO4 amount and PO4 to protein ratio for ODM. X-ray diffraction indicated that mineral produced with elevated Ca2+ in both complete growth and osteogenic differentiating medium had a crystalline structure characteristic of hydroxyapatite. Ionic calcium should be considered a potent regulator in hASC mineralization and could serve as a potential treatment for inducing prompt ossification of hASC-seeded scaffolds for bone tissue engineeri! ng prior to implantation. PMID: 20088702 [PubMed - as supplied by publisher]   This email was sent to agupta1213+termsc@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.