| Engineering of large cartilaginous tissues through the use of microchanneled hydrogels and rotational culture. Tissue Eng Part A. 2009 Nov;15(11):3213-20 Authors: Buckley CT, Thorpe SD, Kelly DJ The development of functional engineered cartilaginous tissues of sufficient size that can be used clinically to treat large defects remains a major and significant challenge. This study investigated if the introduction of microchannels into chondrocyte-seeded agarose hydrogels would result in the formation of a superior and more homogenous cartilaginous tissue as a result of enhanced nutrient transport. Microchanneled construct cylinders were fabricated v! ia a molding process utilizing a pillared structure to create the required architecture. Constructs were subjected to either constant rotation in a rotational bioreactor system or free-swelling conditions. After 28 days of free-swelling culture the presence of microchannels did not enhance glycosaminoglycan accumulation within the core of the construct compared to solid constructs (0.317 +/- 0.002% w/w vs. 0.401 +/- 0.020% w/w). However, under dynamically rotating conditions, glycosaminoglycan accumulation in the cores (1.165 +/- 0.132% w/w) of microchannel constructs were similar to that in the periphery (1.23 +/- 0.074% w/w) of solid constructs, although still significantly lower than their corresponding periphery (1.64 +/- 0.133% w/w) after 28 days. These results confirm that cellular nutrient consumption is primarily responsible for creating the spatial gradients in molecules regulating the biosynthetic activity of chondrocytes through the volume of hydrogels, and that ! changing the scaffold architecture alone may have little effec! t while the inherent diffusivity of the material remains high. Rather, a combination of forced convection and modified scaffold architecture is necessary to engineer large cartilaginous tissues in vitro. PMID: 19374490 [PubMed - indexed for MEDLINE] | 
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