| Non-invasive imaging of bone specific collagen I expression in a luciferase transgenic mouse model. Tissue Eng Part C Methods. 2010 Mar 10; Authors: Liu J, Arends R, Martens AC, den Brok-Bardoel MH, Scheepers MG, van Blitterswijk C, De Boer J Luciferase transgenic mice are a very promising tool for non-invasive, quantitative and longitudinal evaluation of gene expression. The aim of this study was to validate the Col I-Luc transgenic mouse model in which the luciferase gene is driven by bone specific regulatory elements from the mouse collagen alpha (1) I gene for bioluminescent imaging (BLI) of bone development and remodelling. We observed strong! luciferase activity in skeletal tissues of Col(I)-Luc mice, and observed that the light intensity declined with postnatal bone development. Luciferase activity was enhanced in a tail bone repair model and we were able to monitor the process of ectopic bone formation induced by recombinant human bone morphogenetic protein 2 using BLI. We conclude that Col(I)-Luc transgenic mice can be applied in the field of bone tissue engineering for monitoring bone repair processes and to investigate osteoinductive molecules or scaffolds. PMID: 20218816 [PubMed - as supplied by publisher] | 
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