3/7 pubmed: adipose stem cell

Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: adipose stem cell Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells. March 6, 2010 at 7:20 AM Related Articles Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells. Blood. 2010 Feb 4;115(5):957-64 Authors: Han J, Koh YJ, Moon HR, Ryoo HG, Cho CH, Kim I, Koh GY The stromal vascular fraction (SVF) in adipose tissue contains a pool of various stem and progenitor cells, but the existence of hematopoietic stem and progenitor cells (HSPCs) in the SVF has not been seriously considered. We detected the presence of HSPCs in the SVF by phenotypically probing with Lin(-)Sca-1(+)c-kit(+) (LSK) and functionally confirming the presence using colony-forming cell assay and assessing the long-term multilineage reconstitution ability after SVF transplantation. The LSK population in the SVF was 0.004% plus or minus 0.001%, and 5 x 10(5) freshly isolated SVF cells gave rise to 13 plus or minus 4 multilineage colonies. In addition, 0.15% plus or minus 0.03% of SVF cells was home to bone marrow (BM), especially near vascular and endosteal regions, 24 hours after blood transplantation. SVF transplantation was capable of generating a long-term (> 16 weeks), but variable extent (2.1%-32.1%) multilineage reconstitution in primary recipients, ! which was subsequently transferred to the secondary recipients by BM transplantation. All HSPCs within the SVF originated from the BM. Furthermore, the granulocyte-colony-stimulating factor (G-CSF) mobilization of HSPCs from BM markedly elevated the number of phenotypic and functional HSPCs in the SVF, which induced a high efficiency long-term reconstitution in multilineage hematopoiesis in vivo. Our results provide compelling evidence that adipose tissue is a novel extramedullary tissue possessing phenotypic and functional HSPCs. PMID: 19897586 [PubMed - indexed for MEDLINE]   Fighting fat with fat: the expanding field of adipose stem cells. March 6, 2010 at 7:20 AM Related Articles Fighting fat with fat: the expanding field of adipose stem cells. Cell Stem Cell. 2009 Nov 6;5(5):472-81 Authors: Zeve D, Tang W, Graff J We are in the midst of a dire, unprecedented, and global epidemic of obesity and secondary sequelae, most prominently diabetes and hyperlipidemia. Underlying this epidemic is the most hated of cells, adipocytes, and their inherent dynamic ability to expand and renew. This capacity highlights a heretofore undefined stem compartment. Recent in vivo studies, relying upon lineage tracing and flow cytometry methods, have begun to unravel the identity of adipose stem cells, their niche, and the dynamism central to adipose expansion. Thus, the field is moving in a direction that may allow us to manipulate adipose stem cells to beneficial therapeutic ends. PMID: 19896439 [PubMed - indexed for MEDLINE]   Correlation of rapid phenotypic changes and insulin production of differentiated human adipose tissue-derived stem cells. March 6, 2010 at 7:20 AM Related Articles Correlation of rapid phenotypic changes and insulin production of differentiated human adipose tissue-derived stem cells. Ann Plast Surg. 2009 Oct;63(4):436-40 Authors: Scholz T, Satyanarayan S, Dhar S, Evans GR The study investigates adipose tissue-derived stem cells (ADSCs) to differentiate into insulin-secreting cells in vitro. ADSCs were exposed to differentiation medium or control medium. Culture medium was harvested for quantification of insulin and cells were classified into stages of differentiation ranging from normal appearance to islet-like clusters. Morphologic analysis demonstrated marked phenotypic changes towards the islet cell lineage. Thirty-six percent of cells exposed to differentiation medium had progressed to morphologic end-stage after 15 days. Chemiluminescence of the culture medium determined that insulin secretion by differentiated cells progressively increased, reaching a maximum at day 7. Insulin secretion by control cells was significantly less at all time points. A high correlation of secreted insulin and the presence of stage 3 cells were observed throughout the entire experiment. ADSCs can be differentiated into insulin-secreting cells in re! sponse to defined culture conditions. The secreted insulin significantly correlates with phenotypic changes throughout the differentiation process. PMID: 19770705 [PubMed - indexed for MEDLINE]   This email was sent to agupta1213+termsc@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064