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Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. RegenMD A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. March 13, 2010 at 5:15 AM Related Articles A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. Nat Protoc. 2010 Apr;5(4):647-60 Authors: Raya A, Rodríguez-Pizà I, Navarro S, Richaud-Patin Y, Guenechea G, Sánchez-Danés A, Consiglio A, Bueren J, Belmonte JC The generation of patient-specific induced pluripotent stem cells (iPSCs) offers unprecedented opportunities for modeling and treating human disease. In combination with gene therapy, the iPSC technology can be used to generate disease-free progenitor cells of potential interest for autologous cell therapy. We explain a protocol for the reproducible generation of ! genetically corrected iPSCs starting from the skin biopsies of Fanconi anemia patients using retroviral transduction with OCT4, SOX2 and KLF4. Before reprogramming, the fibroblasts and/or keratinocytes of the patients are genetically corrected with lentiviruses expressing FANCA. The same approach may be used for other diseases susceptible to gene therapy correction. Genetically corrected, characterized lines of patient-specific iPSCs can be obtained in 4-5 months. PMID: 20224565 [PubMed - in process]   Nature Blogger Writes About Snyder Grant March 13, 2010 at 5:35 AM   This email was sent to regenmd@gmail.com.  Account Login Don't want to receive this feed any longer? Unsubscribe here This email was carefully delivered by Feed My Inbox. 230 Franklin Road Suite 814 Franklin, TN 37064