|  |  |  | | | | | pubmed: adipose stem cell | | | | | | | | | | | | | | | | | | | In vitro evaluation of polyester-based scaffolds seeded with adipose derived stem cells for peripheral nerve regeneration. J Biomed Mater Res A. 2010 Aug 19; Authors: Tse KH, Sun M, Mantovani C, Terenghi G, Downes S, Kingham PJ To overcome the disadvantages of autografts for peripheral nerve repair, different methods such as artificial nerve conduits have been investigated for an alternative approach. This study demonstrated that solvent casting is a simple but efficient method to create thin polyester-based scaffolds for stem cell delivery. Using poly (epsilon-caprolactone) and poly (D,L-lactic acid), we produced scaffold films containing heterogenous depressions (pits) on the air surface with a size ranging from 0.5 to 30 mum(2). These scaffolds were moderately hydrophobic; however, they supported the differentiation of adipose derived stem cells (ADSC) into a Schwann cell-like phenotype. The differentiated ADSC (dADSC) expressed S100 protein and glial fibrillary acidic protein and readily adhered to the films and proliferated at a similar rate to those cultured on tissue culture polystyrene. Cells were also positive for proliferating cell nuclear antigen. Furthermore, dADSC retained functional activity and significantly enhanced neurite outgrowth from dorsal root ganglia neurons. This study suggests polymer scaffolds combined with dADSCs could be a promising therapy for peripheral nerve injuries. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010. PMID: 20725987 [PubMed - as supplied by publisher] | | | | | | | | | | | | | | | | | | | | | | | | | [Histological and immunohistochemical characteristics of stimulated angiogenesis in transplantation of multipotent stem cells derived from the adipose tissue in patients with chronic ischemia of the extremities] Klin Khir. 2010 May;(5):40-3 Authors: Poliachenko IuV, Driuk MF, Dombrovs'kyÄ DB The cellular technologies application with the objective for the processes of angiogenesis stimulation, in the ischemia conditions, constitutes an actual task for modern stage of a science development, the discovery of a new sources of cells-stimulants for neoangiogenesis is in a progress. The investigation was performed with an objective of studying of histological and immunohistochemical changes in muscular tissue, occurring after autotransplantation of multipotent stromal cells from adipose tissue of the patients, suffering the extremity ischemia. Histological and immunohistochemical investigations (detection of antibodies expression towards Villebrand factor, collagen type IV and vimentin) of processes, occurring in muscular tissue after introduction of multipotent stromal cells from adipose tissue of this patient, were conducted. The reduction of myofibrils ischemic affection and rapid activation of the muscle regenerative potential were shown. There was established a vivid stimulation of the angiogenesis processes during already first month after cellular transplantation performance. PMID: 20623978 [PubMed - indexed for MEDLINE] | | | | | | | | | | | | | | | | | | | | | | | | | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects. J Orthop Res. 2010 Sep;28(9):1193-9 Authors: Oliveira JT, Gardel LS, Rada T, Martins L, Gomes ME, Reis RL In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellan gum with encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASC + GF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Full-thickness articular cartilage defects were created and the gellan gum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASC + GF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASC + GF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cells may constitute a valid alternative to currently used articular chondrocytes. PMID: 20187118 [PubMed - indexed for MEDLINE] | | | | | | | | | |  | |  | |  |
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