| Differentiation of human adipose-derived stem cells induced by recombinantly expressed fibroblast growth factor 10 in vitro and in vivo.
November 17, 2009 at 7:38 am
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| | Differentiation of human adipose-derived stem cells induced by recombinantly expressed fibroblast growth factor 10 in vitro and in vivo. In Vitro Cell Dev Biol Anim. 2009 Nov 14; Authors: Zhang X, Wu M, Zhang W, Shen J, Liu H The adipogenesis effect of fibroblast growth factor 10 (FGF10) has been demonstrated in many studies. The aim of this study is to render a novel method which can continuously induce hypodermal adipose-derived stem cell (ADSC) differentiation and maturation in vivo and in vitro using FGF10. We constructed a recombinant pcDNA3.0-FGF10-MSC which can continuously express FGF10 by transfected FGF10 into a human mesenchymal stem cell (MSC) clone, and we cultured ADSCs from human subcutaneous resected adipose tissue. An in vitro and in vivo co-culture system of pcDNA3.0-FGF10-MSC and ADSCs was then established. We observed the characteristics of ADSCs, monitored the adipogenesis-related transcription factor CAAT/enhancer binding protein-beta, peroxisome proliferator-activated receptor-gamma, and measured the adipose tissue layer of carrier animals. The results showed that FGF10 secreted from pcDNA3.0-FGF10-MSC could induce ADSC differentiation into mature adipocytes consistently. The study demonstrated that FGF10 can promote the adipogenesis effect in situ, and the autotransplantation of a carrier continuously secreting FGF10 may be utilized for increasing local subcutaneous adipose tissue in cosmetology. PMID: 19915940 [PubMed - as supplied by publisher] |
| The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices.
November 17, 2009 at 7:38 am
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| | The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices. Biomaterials. 2009 Nov 13; Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M We investigated the combination of human adipose tissue derived stem cells (ADSC) and in vivo gel-forming methoxy poly (ethyleneglycol)-poly (varepsilon-caprolactone) (MPEG-PCL) as a muscle regeneration matrix, with and without inclusion of vascular endothelial cell growth factor (VEGF). VEGF(165)-treated stem cell grafts showed significant proliferation and differentiation into muscle tissue in vivo. Importantly, the inclusion of VEGF enhanced vascularization. This scaffold supported preconditioned ADSC, and allowed them to differentiate into mature muscle tissues in vivo, indicating that ADSC of human origin and MPEG-PCL scaffolds provided an appropriate environment for cellular growth and expansion. Our results thus provide a potential solution to the major obstacle encountered in the engineering of thick complex tissues, which require an adequate blood supply to maintain cell viability during tissue growth and to induce appropriate structural organization. Therefore, the combination of ADSC and in vivo gel-forming MPEG-PCL with VEGF(165) might serve as a suitable non-invasive biomaterial for clinical muscle regeneration applications. PMID: 19914711 [PubMed - as supplied by publisher] |
| [Nurse involvement in clinical trials with cell therapy.]
November 17, 2009 at 7:38 am
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| | [Nurse involvement in clinical trials with cell therapy.] Enferm Clin. 2009 Nov 12; Authors: de la Quintana Jiménez P Research with stem cells has made a qualitative leap since the research group from Minnesota University Stem Cell Institute in the USA published a study in July 2002, which showed that the bone marrow stem cells of adults multiplied indefinitely without losing their ability to differentiate. In this hospital the first implant of stem cells derived from adipose tissue was performed in this hospital in 2003 in an attempt to close a complex perianal fistula in a patient with Crohn's disease. As a result of this first success, a multidisciplinary research team was formed to study the application of stem cells in the treatment of complex anal fistula. Since its creation, it has included a nurse whose role is fundamental in the coordination of the different phases of the trials and the involvement of patients in these. The development of these new treatments, along with their complexity and direct effects on the patient, involves the training and presence of the professional nurse in these teams. The improvement shown in tissue healing would make it very worthwhile to demonstrate their efficacy in wound care, pressure sores, diabetic foot, etc. PMID: 19914112 [PubMed - as supplied by publisher] |
| [Induction of hepatic specification of human adipose-derived stem cells (hADSCs) in vitro.]
November 17, 2009 at 7:38 am
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| | [Induction of hepatic specification of human adipose-derived stem cells (hADSCs) in vitro.] Zhonghua Gan Zang Bing Za Zhi. 2009 Jul;17(7):544-8 Authors: Wang M, Pei HY, Guan LD, Nan X, Bai CX, Liu H, Li BW, Wang YF, Pei XT OBJECTIVE: To induce hepatic differentiation of human adipose-derived stem cells (hADSCs) in vitro. METHODS: hADSCs were isolated from human adipose tissue and treated with improved hepatic medium containing HGF, bFGF and FGF4. After 7 days of culture, OSM was added to the culture media. Cell growth during hepatic differentiation was evaluated by CCK8 assay. Morphology of differentiation was examined under light microscope. Liver specific genes and proteins were detected by RT-PCR analysis and immunohistochemical staining, respectively. And functional characteristics of hepatocytes were also examined. RESULTS: The number of hADSCs cultured in the improved hepatic media was increased significantly in comparison to hADSCs cultured in control media from 5 days to 21days (t = 6.59, 8.69, 15.94 and 24.64, respectively, P less than 0.05). The hADSCs-derived hepatocyte-like cells exhibited hepatocyte morphology, expressed hepatocyte markers, possessed hepatocyte-specific activities, such as uptake and excretion of indocyanine green, glycogen storage and albumin production. CONCLUSION: hADSCs can be induced into hepatocyte-like cells in this differentiation system. And this differentiation system promoted the growth of hADSCs. DOI: 10.3760/cma.j.issn.1007-3418.2009.07.017. PMID: 19912692 [PubMed - in process] |
| Application of mesenchymal stromal cells in urological diseases.
November 17, 2009 at 7:38 am
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| | Application of mesenchymal stromal cells in urological diseases. BJU Int. 2009 Nov 13; Authors: Montzka K, Heidenreich A The application of stem cells and their use in tissue-engineering approaches is emerging in clinical therapeutic intervention strategies. The use of adult stem cells, either autologous or allogenic, does not raise ethical concerns, in contrast to embryonic stem cells. Mesenchymal stromal cells (MSCs) can be easily obtained from bone marrow or from adipose tissue and further expanded in vitro. Due to their differentiation capacity, MSCs are very attractive for tissue engineering purposes. Furthermore, MSCs secrete a variety of mediators that have beneficial effects on the regenerating tissue. In this review we give an insight into stem cell hierarchy, define the properties of MSCs and summarize recent reports of their administration in urological diseases. PMID: 19912199 [PubMed - as supplied by publisher] |
| Adult stem cells in tissue engineering.
November 17, 2009 at 7:38 am
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| | Adult stem cells in tissue engineering. Expert Rev Med Devices. 2009 Nov;6(6):621-40 Authors: Hodgkinson T, Yuan XF, Bayat A Tissue engineering is a rapidly evolving field of research that has yet to fulfil its promise in the translation and potential application of adult stem cells in clinical practice. Recently, it has become apparent that specific adult stem cells are capable of transdifferentiation. The successful application of adult stem cells is thought to be central in creating truly biomimetic tissue. Although still most widely utilized, research suggests that in the future, bone marrow-derived stem cells may no longer be considered the most suitable candidates for use in tissue engineering. Independent studies have successfully engineered a range of tissues in vitro and in vivo using hair follicle- and adipose-derived stem cells. Owing to their potency, relative abundance and noninvasive extraction, these populations may be the most promising studied to date. This review aims to discuss these candidate adult stem cell populations in an attempt to assess the most promising avenues of research. PMID: 19911874 [PubMed - in process] |
| Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.
November 17, 2009 at 7:38 am
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| | Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. J Exp Med. 2009 Oct 26;206(11):2483-96 Authors: Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H, Matsuzaki Y Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRalpha+Sca-1+CD45-TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities. PMID: 19841085 [PubMed - indexed for MEDLINE] |
| Adipose-derived cardiomyogenic cells: in vitro expansion and functional improvement in a mouse model of myocardial infarction.
November 17, 2009 at 7:38 am
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| | Adipose-derived cardiomyogenic cells: in vitro expansion and functional improvement in a mouse model of myocardial infarction. Cardiovasc Res. 2009 Sep 1;83(4):757-67 Authors: Léobon B, Roncalli J, Joffre C, Mazo M, Boisson M, Barreau C, Calise D, Arnaud E, André M, Pucéat M, Pénicaud L, Prosper F, Planat-Bénard V, Casteilla L AIMS: Cells derived from the stroma vascular fraction (SVF) of mouse adipose tissue can spontaneously give rise to rare, functional, cardiac-like cells in vitro. This study aimed to improve the production of adipose-derived cardiomyogenic cells (AD-CMG), to characterize them and to assess their cardiac fate and functional outcomes after their administration in a mouse model of acute myocardial infarction. METHODS AND RESULTS: The culture process optimized to improve in vitro cardiac specification consisted of a primary culture of murine SVF cells in semi-solid methylcellulose medium, a selection of AD-CMG cell clusters, and a secondary culture and expansion in BHK21 medium. AD-CMG cells were CD29(+), CD31(-), CD34(-), CD44(+), CD45(-), CD81(+), CD90(-), CD117(-), and Flk-1(-) and expressed several cardiac contractile proteins. After 1, 2, and 4 weeks of their injection in mice having acute myocardial infarction, a strong presence of green fluorescent protein-positive cells was identified by immunohistochemistry as well as quantitative polymerase chain reaction. Echocardiography showed a significant reduction of remodelling and stability of left ventricle ejection fraction in the AD-CMG cell-treated group vs. controls. Vascular density analysis revealed that AD-CMG administration was also associated with stimulation of angiogenesis in peri-infarct areas. CONCLUSION: Cardiomyogenic cells can be selected and expanded in large amounts from mouse adipose tissue. They can survive and differentiate in an acute myocardial infarction model, avoiding remodelling and impairment of cardiac function, and can promote neo-vascularization in the ischaemic heart. PMID: 19505931 [PubMed - indexed for MEDLINE] |
| Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model.
November 17, 2009 at 7:38 am
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| | Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model. Cytotherapy. 2009;11(3):289-98, 1 p following 298 Authors: Sun B, Roh KH, Park JR, Lee SR, Park SB, Jung JW, Kang SK, Lee YS, Kang KS BACKGROUND AIMS: Mesenchymal stromal cells (MSC) have been studied intensively in regenerative medicine. However, their therapeutic potential against tumor formation and cancer metastasis is still unclear. The effects of transplantation of MSCs in early-stage of carcinogenesis, should be evaluated. METHODS: MSC isolated from human umbilical cord blood (UCB) and adipose tissue (AD) were transplanted in a mouse cancer metastasis model. The effects of MSC on tumor growth and metastasis were analyzed. The effects of transplantation of MSC into the mouse model at very early stage carcinogenesis were also evaluated. RESULTS: Human MSC reduced lung metastasis and inhibited the growth of human breast cancer cells by inducing apoptosis. In addition, transplantation of both UCB and AD MSC into a cancer model with no detectable clinical symptoms did not appear to promote tumor growth or metastasis. CONCLUSIONS: We evaluated the effect of MSC derived from human UCB and AD tissue in a tumor model. Our findings may help to elucidate the interaction between cancer cells and MSC, as well as the application of MSC to clinical trials. PMID: 19308770 [PubMed - indexed for MEDLINE] | | | 
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