| 2 children suffering from adrenoleukodystrophy saved thanks to the ELA Association
November 5, 2009 at 7:23 pm
|
| |
| ThermoGenesis Reports 15 Percent Increase in First Quarter Revenues; Improved Bottom Line
November 5, 2009 at 5:22 pm
|
| |
| Jonathan Moreno and Andrew Light to Speak About Controversies in Biomedical and Environmental Science and Policy at The National Press Club on November 09, 2009
November 5, 2009 at 12:22 pm
|
| |
| Stem Cell Therapy International, Inc. and Histostem Korea Announce Providing Hematopoietic Stem Cell to Queen Mary Hospital, Hong Kong
November 5, 2009 at 11:22 am
|
| |
| StemCells, Inc. Reports Third Quarter Financial Results
November 5, 2009 at 10:21 am
|
| |
| Beike Biotechnology Invests in Leading Japanese Regenerative Medicine Co., Biomaster Inc.
November 5, 2009 at 10:21 am
|
| |
| SpineSmith Partners and GenOsteo Announce Collaborative Product Development Agreement
November 5, 2009 at 10:21 am
|
| |
| Cadherin and integrin regulation of epithelial cell migration.
November 5, 2009 at 6:29 am
|
| | Cadherin and integrin regulation of epithelial cell migration. Langmuir. 2009 Sep 1;25(17):10092-9 Authors: Silvestre J, Kenis PJ, Leckband DE These studies quantified the relative effects of E-cadherin expression and homophilic ligation on the integrin-mediated motility of epithelial cells. Micropatterned proteins were used to quantitatively titrate the ligation of E-cadherin and integrin receptors in order to assess their coordinate influence on the migration velocities of MDA-MB-231 breast tumor epithelial cells. Fibronectin, E-cadherin, and mixtures of fibronectin and E-cadherin were covalently patterned on solid surfaces at defined compositions and mass coverages. The migration velocities of parental epithelial cells and of cells engineered to express E-cadherin under tetracycline control show that E-cadherin expression reduces cell motility by both adhesion-dependent and adhesion-independent mechanisms. Increasing E-cadherin expression levels also suppresses the dependence of cell velocity on the fibronectin coverage. On E-cadherin-containing substrata, the cell velocity decreases both with the E-cadherin expression level and with the immobilized E-cadherin surface density. These studies thus identified conditions under which E-cadherin preferentially suppresses cell migration by adhesion-independent versus adhesion-dependent mechanisms. PMID: 19583181 [PubMed - indexed for MEDLINE] |
| In vitro effects of nanophase hydroxyapatite particles on proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells.
November 5, 2009 at 6:29 am
|
| | In vitro effects of nanophase hydroxyapatite particles on proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. J Biomed Mater Res A. 2009 Sep 15;90(4):1083-91 Authors: Liu Y, Wang G, Cai Y, Ji H, Zhou G, Zhao X, Tang R, Zhang M Coculturing scaffolds with seeded cells in vitro is an indispensable process for construction of engineered tissues. It is essential to understand effects of the constituent particles of scaffold on seeded cells. In this study, we investigated the influence of nano-sized hydroxyapatite (nHAP) particles on the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs). nHAP particles were cocultured with MSCs separated from rabbit. Cellular effects of particles were determined by cell counts, Vonkossa stains, and reverse transcription-polymerase chain reaction (RT-PCR) examinations. Results showed that nHAP particles could promote the MSCs growth when particle concentrations were lower than 20 microg/10(4) cells. This effect disappeared when the particles and the cells were cocultured in serum-free media. Higher particle concentrations could significantly inhibit the cell growth. Under the standard culture condition, the only effect of nHAP particles on osteogenic differentiation of MSCs was to enhance the expression of collagen I. Under the osteogenic-inductive culture condition, nHAP particles could inhibit mineralization of cells but promote their osteogenic differentiation. These cellular effects of particles still existed when the particles and the cells were cultured in indirect coculture system. nHAP particles could decrease calcium and phosphate concentrations of culture media, which possibly contributed to the cellular effects of nHAP particles. PMID: 18671263 [PubMed - indexed for MEDLINE] |
| Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation.
November 5, 2009 at 6:29 am
|
| | Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation. J Biomed Mater Res A. 2009 Sep 15;90(4):999-1009 Authors: Bush KA, Driscoll PF, Soto ER, Lambert CR, McGimpsey WG, Pins GD Precisely engineering the surface chemistry of biomaterials to modulate the adsorption and functionality of biochemical signaling molecules that direct cellular functions is critical in the development of tissue engineered scaffolds. Specifically, this study describes the use of functionalized self-assembled monolayers (SAMs) as a model system to assess the effects of biomaterial surface properties on controlling fibronectin (FN) conformation and concentration as well as keratinocyte function. By systematically analyzing FN adsorption at low and saturated surface densities, we distinguished between SAM-dependent effects of FN concentration and conformation on presenting cellular binding domains that direct cellular functions. Quantitative image analyses of immunostained samples showed that modulating the availability of the FN synergy site directly correlated with changes in keratinocyte attachment, spreading, and differentiation, through integrin-mediated signaling mechanisms. The results of this study will be used to elucidate design features that can be incorporated into dermal equivalents and percutaneous implants to enhance the rate of re-epithelialization and tissue regeneration. Furthermore, these findings indicate that SAM-based model systems are a valuable tool for designing and investigating the development of scaffolds that regulate the conformation of extracellular matrix cues and cellular functions that accelerate the rate of tissue regeneration. PMID: 18655147 [PubMed - indexed for MEDLINE] |
| [Combination of reconstructive vascular operations with gene-engineering technologies of angiogenesis stimulation: a present-day policy aimed at improving the remote results of treating patients with lower limb chronic ischaemia]
November 5, 2009 at 6:29 am
|
| | [Combination of reconstructive vascular operations with gene-engineering technologies of angiogenesis stimulation: a present-day policy aimed at improving the remote results of treating patients with lower limb chronic ischaemia] Angiol Sosud Khir. 2008;14(4):49-53 Authors: Gavrilenko AV, Voronov DA, Konstantinov BA, Bochkov NP The authors have studied therapeutic outcomes in a total of 38 patients diagnosed with occlusions of the femoropopliteal segment. In the Study Group patients (n = 19), the operation of femoropopliteal bypass grafting was supplemented by using gene stimulators of angiogenesis (gene constructions with the genes of vascular endothelial growth factor, and angiogenin). The Control Group patients (n = 19) were subjected to a reconstructive vascular operation alone. The remote results were followed up from six to twenty-six months, having shown reliably better therapeutic outcomes obtained in the Study Group patients, as judged by the distance of pain-free walking, the time of restoration of the baseline parameters of blood flow during the treadmill test, muscular perfusion, and the quality of life indices. A conclusion was made that the use ofangiogenesis-stimulating methods combined with reconstructive vascular operations improves the long-term outcomes in patients presenting with lower limb chronic ischaemia. PMID: 19791552 [PubMed - indexed for MEDLINE] |
| Innovation in basic science: stem cells and their role in the treatment of paediatric cardiac failure--opportunities and challenges.
November 5, 2009 at 6:02 am
|
| | Innovation in basic science: stem cells and their role in the treatment of paediatric cardiac failure--opportunities and challenges. Cardiol Young. 2009 Nov;19 Suppl 2:74-84 Authors: Kaushal S, Jacobs JP, Gossett JG, Steele A, Steele P, Davis CR, Pahl E, Vijayan K, Asante-Korang A, Boucek RJ, Backer CL, Wold LE Heart failure is a leading cause of death worldwide. Current therapies only delay progression of the cardiac disease or replace the diseased heart with cardiac transplantation. Stem cells represent a recently discovered novel approach to the treatment of cardiac failure that may facilitate the replacement of diseased cardiac tissue and subsequently lead to improved cardiac function and cardiac regeneration. A stem cell is defined as a cell with the properties of being clonogenic, self-renewing, and multipotent. In response to intercellular signalling or environmental stimuli, stem cells differentiate into cells derived from any of the three primary germ layers: ectoderm, endoderm, and mesoderm, a powerful advantage for regenerative therapies. Meanwhile, a cardiac progenitor cell is a multipotent cell that can differentiate into cells of any of the cardiac lineages, including endothelial cells and cardiomyocytes. Stem cells can be classified into three categories: (1) adult stem cells, (2) embryonic stem cells, and (3) induced pluripotential cells. Adult stem cells have been identified in numerous organs and tissues in adults, including bone-marrow, skeletal muscle, adipose tissue, and, as was recently discovered, the heart. Embryonic stem cells are derived from the inner cell mass of the blastocyst stage of the developing embryo. Finally through transcriptional reprogramming, somatic cells, such as fibroblasts, can be converted into induced pluripotential cells that resemble embryonic stem cells. Four classes of stem cells that may lead to cardiac regeneration are: (1) Embryonic stem cells, (2) Bone Marrow derived stem cells, (3) Skeletal myoblasts, and (4) Cardiac stem cells and cardiac progenitor cells. Embryonic stem cells are problematic because of several reasons: (1) the formation of teratomas, (2) potential immunologic cellular rejection, (3) low efficiency of their differentiation into cardiomyocytes, typically 1% in culture, and (4) ethical and political issues. As of now, bone marrow derived stem cells have not been proven to differentiate reproducibly and reliably into cardiomyocytes. Skeletal myoblasts have created in vivo myotubes but have not electrically integrated with the myocardium. Cardiac stem cells and cardiac progenitor cells represent one of the most promising types of cellular therapy for children with cardiac failure. PMID: 19857353 [PubMed - in process] | | | 
|
No comments:
Post a Comment