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Please add updates@feedmyinbox.com to your address book to make sure you receive these messages in the future. pubmed: "regenerative medici... Up-regulation in the expression of renin gene by the influence of aluminium. September 9, 2009 at 8:27 am Related Articles Up-regulation in the expression of renin gene by the influence of aluminium. J Inorg Biochem. 2009 Aug 14; Authors: Ezomo OF, Matsushima F, Meshitsuka S The excretion of aluminium in urine was significantly increased after intake of analgesics containing aluminium, confirming increased absorption and hence exposure to aluminium with such medication. The effect of aluminium on the kidney was further investigated by study of gene expression in mice. After a single dose of aluminium, an up-regulation of renin gene was found by DNA sequencing of the products of differential display analysis. The up-regulation of renin was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting experiments in the dose dependent treatments and the time course observation after aluminium citrate injection. The up-regulation of the renin expression by aluminium is a strong indication of the influence of aluminium on the renin-angiotensin-aldosterone-system, resulting in possible induction of essential hypertension. PMID: 19735944 [PubMed - as supplied by publisher] Total saponins of Panax ginseng (TSPG) promote erythroid differentiation of human CD34(+) cells via EpoR-mediated JAK(2)/STAT(5) signaling pathway. September 9, 2009 at 8:27 am Related Articles Total saponins of Panax ginseng (TSPG) promote erythroid differentiation of human CD34(+) cells via EpoR-mediated JAK(2)/STAT(5) signaling pathway. J Ethnopharmacol. 2009 Sep 4; Authors: Chen D, Zuo G, Li C, Hu X, Guan T, Jiang R, Li J, Lin X, Li F, Luo C, Wang H, Lei C, Long X, Wang Y, Wang J ETHNOPHARMACOLOGICAL RELEVANCE: Total saponins of Panax ginseng (TSPG), main constituents extracted from Panax ginseng, a highly valued traditional Chinese medicine, have been shown to be an effective agent on hematopoiesis. OBJECTIVE: To investigate the effect and mechanism underlying which TSPG promote human CD34(+) hematopoietic stem and progenitor cells to differentiate into erythroid lineage cells. MATERIALS AND METHODS: The effect of TSPG on erythroid differentiation of purified CD34(+) cells derived from umbilical cord blood (UCB) was determined by methylcellulose assay system and colorimetry for hemoglobin content. The changes of EpoR expression in umbilical cord blood mononuclear cells (UCB-MNCs) and purified CD34(+) cells were detected with Western blotting and flow cytometry, respectively, and observed under Laser Scanning Confocal Microscope (LSCM). RT-PCR was performed to examine EpoR mRNA expression in CD34(+) cells. The effects of TSPG-pretreatment on Epo-induced JAK(2) and STAT(5) tyrosine phosphorylation were analyzed by immunoprecipitation. RESULTS: The addition of TSPG (20-70mg/L) increased the colony formation rate of BFU-E. TSPG (50mg/L) alone used significantly increased the hemoglobin content, the addition of AG490 evidently reduced TSPG-induced elevation of hemoglobin content. TSPG increased expression of EpoR on the surface membrane of CD34(+) cells but did not change the expression of EpoR in total UCB-MNCs. TSPG also increased expression of EpoR mRNA in CD34(+) cells. TSPG markedly enhanced Epo-induced tyrosine phosphorylation of JAK(2) and STAT(5) in UCB-MNCs. CONCLUSION: These findings suggest that TSPG may enhance the erythroid differentiation of hematopoietic stem and progenitor cells via Epo/EpoR-mediated JAK(2) / STAT(5) signaling pathway. PMID: 19735711 [PubMed - as supplied by publisher] Quantitative analysis of tumor-derived methylated RUNX3 sequences in the serum of gastric cancer patients. September 9, 2009 at 8:27 am Related Articles Quantitative analysis of tumor-derived methylated RUNX3 sequences in the serum of gastric cancer patients. Anticancer Res. 2009 Jul;29(7):2619-25 Authors: Sakakura C, Hamada T, Miyagawa K, Nishio M, Miyashita A, Nagata H, Ida H, Yazumi S, Otsuji E, Chiba T, Ito K, Ito Y PURPOSE AND EXPERIMENTAL DESIGN: Using real-time quantitative methylation-specific PCR (RTQ-MSP), methylated RUNX3 sequences were quantified and the fractional concentrations of circulating tumor DNA in serum were determined, along with peripheral blood cells collected preoperatively, intraoperatively and postoperatively from 65 patients with gastric cancer. RESULTS: RTQ-MSP was sufficiently sensitive to detect RUNX3 methylation. Quantitative MSP data were expressed in terms of the methylation index, which was defined as the relative amount of methylated RUNX3 sequences divided by the concentration of methylated actin. High levels of methylated RUNX3 sequences were detected in the peripheral circulation of 29% (19 of 65) of the gastric cancer patients. The RUNX3 methylation index was concordant with cancer stage, histology, lymphatic and vascular invasion, and was more sensitive than carcinoembryonic antigen (CEA) as a biomarker. Twenty-nine percent (19 out of 65) of preoperative serum samples had methylated RUNX3 sequences, ranging from 5.2 to 1625955 (median quantity=43 m-index, sensitivity 95.5%, specificity 62.5%, AUC 0.8651). After surgical resection, the median RUNX3 methylation index in serum significantly decreased. These results demonstrate the clinical usefulness and effectiveness of peripheral blood RTQ-MSP for detecting and monitoring gastric cancer after treatment. Furthermore, 5 out of the 30 preoperative control samples of benign disease (cases of panperitonitis due to acute appendicitis or cholecystitis) showed transient RUNX3 methylation which decreased after the operation in accordance with recovery. CONCLUSION: Quantification of epigenetic changes in serum RUNX3 methylation using RTQ-MSP is useful for the detection and monitoring of gastric cancer. PMID: 19596937 [PubMed - indexed for MEDLINE]   This email was sent to agupta1213+termsc@gmail.com.  Manage Your Account Don't want to receive this feed any longer? Unsubscribe here.