| 3D hydrogel model using adipose-derived stem cells for vocal fold augmentation. Tissue Eng Part A. 2009 Sep 3; Authors: Park H, Karajanagi S, Wolak K, Aanestad J, Deheron L, Kobler J, Lopez-Guerra G, Heaton J, Langer RS, Zeitels S Adipose-derived stem cells (ASCs) may provide a clinical option for rebuilding damaged superficial lamina propria (SLP) of the vocal fold. We investigated the effects of five hydrogels (hyaluronic acid (HA), collagen, fibrin, and co-gels of fibrin-collagen and fibrin-HA) on the differentiation of ASCs, with the long term goal of establishing the conditions necessary for controlling the differentiation of ASC into the functional equivalent of SLP fibroblasts. Human ASCs were isolated and characterized by FACS and real time PCR. According to FACS and gene analysis, over 90% of isolated ASCs expressed adult stem cell surface markers and expressed adult stem cell genes. Scaffold-specific gene expression and morphology were assessed by culturing the ASCs in 3-D hydrogels. Two-fold higher amounts of total DNA were detected in fibrin and co-gel cultures compared to collagen and HA cultures. Elastin expression was significantly higher in cells grown in fibrin-based gels compared to cells grown in other gels. Cells grown in the co-gels showed elongated morphology, expressed decorin marker and glycosaminoglycan synthesis, which indicate ASC differentiation. Our data suggest that it may be possible to control the differentiation of ASCs using scaffolds appropriate for vocal fold tissue engineering applications. In particular, co-gels of HA or collagen with fibrin enhanced proliferation, differentiation and elastin expression. PMID: 19728785 [PubMed - as supplied by publisher] | 
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